Effect of Shengmai power on VEGF expression of pancreatic β cells in type 2 diabetic rats
WU Xiaohui
LIU Fei
DUAN Zhongxin
Abstract：Objective To observe the regulating effect of Shengmai powder on the expression levels of nuclear factor-κB/inhibitor nuclear factor-κB (NF-κB/IκB) and vascular endothelial growth factor (VEGF) in the pancreaticβcells of rats with type 2 diabetes mellitus (T2DM).Methods Totally 120 SD male rats were randomly divided into the groups A, B, C, D, E, and F.Rats in the group A were given basic diet, while rats in the other groups (B, C, D, E and F) were fed with high-fat diet combined with intraperitoneal injection of low-dose streptozotocin (STZ) to establish the rat models of diabetic obesity.After 8 weeks, the successfully established rat models were selected from each group.Rats in the group B were given the same amount of normal saline by gavage, group C 0.2 g/ (kg·d) aspirin, and groups D, E and F were given 4.7, 9.4 and 18.8 g/ (kg·d) Shengmai powder.All treatments lasted for 4 weeks.Fasting blood glucose (FBG), fasting insulin (FINS), insulin sensitivity index (ISI) and insulin resistance (HOMA-IR) were measured before and after the above intervention.In addition, serum VEGF was detected by enzyme-linked immunosorbent assay (ELASA), the change of vascular wall structure was observed by HE staining, and NF-κB and IκB mRNA in isletβcells of rats were detected by real-time PCR.Results After 4 weeks of treatment, HE staining showed that the change of vascular wall structure was ameliorated and accompanied with tendency of repair in the groups D, E and F, especially in the latter two groups (P＜0.05).Compared with the group B, the levels of FBG, FINS, and HOMA-IR decreased in the groups D, E and F, while the level of ISI increased.Compared with the group C, the levels of FBG and FINS were higher in the group D, the levels of FBG, FINS, and HOMA-IR were lower, and the level of ISI was higher in the groups E and F, with statistically significant difference (all P＜0.05).Compared with the group B, the levels of NF-κB mRNA and serum VEGF decreased significantly in the groups C, D, E, and F, and the levels of IκB mRNA in the groups C, D, E and F increased significantly (P＜0.05).Conclusion Shengmai powder can significantly decrease the level of insulin resistance and VEGF in serum by inhibiting the serum NF-κB/IκB expression.
Keywords：type 2 diabetes mellitusShengmai powderinsulin resistancenuclear factor-κB/inhibitor nuclear factor-κBvascular endothelial growth factor
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 45-48 )