Effect of Cdk5 inhibitor Roscovitine on pain behavior of rats with neuropathic pain
ZHONG Yu
CHEN Jialin
XIE Yubo
CHEN Jing
Abstract：Objective To evaluate the effect and mechanism of Cdk5 inhibitor Roscovitine on pain behavior of rats with neuropathic pain.Methods Sixty-four SPF Sprague-Dawley male rats were randomly divided into 5 groups:the normal control group (group N, n=8), sham operation group (group S, n=8), chronic constriction injury of sciatic nerve (CCI) group (group I, n=8), CCI+solvent (group D, n=8), and CCI+Roscovitine (group R, n=32).Neuropathic pain was produced by CCI in rats at 7 days after catheterization of the spinal subarachnoid space.Rats in the group N did not receive any treatment, the sciatic nerve of rats in the group S was exposed but was not ligated.5％DMSO (20μL) was injected intrathecally into the rats of the group D, and 100 ug Roscovitine (in 20μL 5％DMSO) was injected intrathecally into the rats of group R at 7 days after CCI.At 1 d (T0) before CCI, 3 d (T1), 5 d (T2) and 7 d (T3) after CCI, and 0.5 h (T4), 2 h (T5), 6 h (T6) and 12 h (T7) after administration, the paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were all measured.Rats in the groups N, S, and I were sacrificed on day 7 after CCI without intrathecal injection.Rats in the group R were sacrificed at 0.5, 2, 6 and 12 h after intrathecal injection on day 7 after CCI.Rats in the group D were sacrificed after intrathecal injection of 5％DMSO (20μL) on day 7after CCI.The expression of GFAP in the spinal cord astrocytes was determined by Western blotting.Results Compared with the group S, PWMT and PWTL in the group I, group D, and group R decreased at different time points after operation (all P＜0.05).Compared with the group D, PWMT increased and PWTL increased during T5 to T7 in the group R (P＜0.05).Compared with the group S, GFAP protein expression in the dorsal horn of spinal cord in the groups I, D, and R at T4, T5, T6, and T7was significantly up-regulated (all P＜0.05).Compare with the group D, GFAP expression in dorsal horn astrocytes of spinal cord at T5, T6and T7after intrathecal injection in the group R was significantly down-regulated (P＜0.05).Conclusion Cdk5 inhibitor Roscovitine can alleviate neuropathic pain in rats caused by sciatic nerve ligation, and its mechanism may be related to inhibition of GFAP protein expression.
Keywords：neuropathic painastrocytescyclin-dependent protein kinase 5rats
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 41-44 )