Effect of silencing LncRNA HOTAIR on proliferation and apoptosis of bladder cancer cell line 253J and its mechanism
LIU Hongjian
FENG Yougang
YU Zhou
KANG Yongming
HE Wei
JIANG Mingdong
Abstract：ObjectiveTo observe the effects of silencing long-chain non-coding RNA (LncRNA) HOTAIR on the proliferation and apoptosis of bladder cancer cell line 253J and to explore its mechanism.Methods 253J cells in logarithmic phase were divided into the si HOTAIR interference group, empty vector group, and control group.The cells in the si HOTAIR interference group and empty vector group were transfected with si HOTAIR (which could silence the expression of HOTAIR) and the negative sequence of the empty vector, respectively.No treatment was done in the control group.CCK8assay was used to observe the proliferation of cells in each group after 4-hour transfection, and Hoechst method was used to observe the apoptosis in each group.The mRNA expression of Notch1, Ep CAM, CXCR2, E-cadherin, vimentin, matrix metalloproteinase 2 (MMP-2) andα-SMA in each group was detected by real-time fluorescence quantitative PCR.ResultsAt 4 h after transfection, the OD values of the si HOTAIR interference group, empty vector group and control group were0.38±0.02, 0.89±0.01, and 0.89±0.02, respectively.Compared with the empty vector group and control group, the OD value of the si HOTAIR group decreased (P＜0.05).At 4 h after transfection, the number of apoptotic cells in the si HOTAIR interference group, empty vector group and control group were 33.56±2.26, 13.69±1.25, and 12.85±1.35, respectively.The number of apoptotic cells in the si HOTAIR group was higher than that of the control group and empty vector group (P＜0.05).Compared with the control group and empty vector group, the relative mRNA expression of Notch1, Ep CAM, CXCR2, Vimentin, MMP-2, andα-SMA decreased, but E-cadherin mRNA expression increased in the si HOTAIR interference group (all P＜0.05).ConclusionSilencing HOTAIR expression can inhibit the proliferation and promote the apoptosis of bladder cancer cells by inhibiting the expression of Notch1, Ep CAM, CXCR2, Vimentin, MMP-2, andα-SMA and promoting the expression of E-cadherin.
Keywords：bladder carcinomalong-chain non-coding RNA HOTAIRcell proliferationapoptosismechanism of action
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 37-40 )