Expression and significance of microRNA-17 in Helicobacter pylori-associated gastric cancer
ZHU Yanli
DU Jingfeng
SU Dewang
LIU Liang
CHENG Zhuoxin
ZHANG Xuemei
Abstract：Objective To observe the expression change of microRNA-17 in the helicobacter pylori (Hp) -associated gastric cancer tissues and its clinical significance.MethodsSeventy-five ceases of gastric cancer tissue specimens and their matched adjacent tissue specimens from patients with gastric cancer were collected, including 48 patients with hp-positive gastric cancer and 27 patients with hp-negative gastric cancer.Real-time quantitative PCR was used to determine the expression levels of cytotoxin-related gene A (Cag A) mRNA and miR-17.Results The expression levels of Cag A mRNA in Hp-positive gastric cancer tissues (6.061±0.712) was significantly higher than that in the Hp-negative gastric cancer patients (1.024±0.235) (t=35.593, P＜0.05).The miR-17 expression levels of gastric cancer tissues and adjacent tissues in patients with Hp-positive gastric cancer were significantly higher than those in patients with Hp-negative gastric cancer (12.785±1.324 vs 5.654±0.745, 8.124±1.105 vs 3.124±0.564) (t=23.739, 21.911, P＜0.01).The miR-17 expression level of gastric cancer tissues in the Hp-positive and Hp-negative patients was significantly higher than that in the adjacent tissues (12.785±1.324 vs 8.124±1.105, 5.654±0.745 vs 3.124±0.564) (t=18.725, 14.069, P＜0.01).In the gastric cancer tissues, miR-17 expression was positively correlated with Cag A expression (r=0.564, P＜0.01).The level of miR-17 expression of gastric cancer in patients with tumor diameter>5cm, TNM stagingⅢ-Ⅳ, Borrmann typeⅢ-Ⅳand lymph node metastasis Hp infection was significantly higher than that of patients with tumor diameter less≤5 cm, TNM stageⅠ-Ⅱ, Borrmann typing-Ⅱ, non-lymph node metastasis (15.226±1.325 vs 11.041±1.214, 14.485±1.412 vs 11.571±1.1.16, 14.341±1.325 vs 11.468±1.241, 15.124±1.332 vs 9.778±1.123), with statistically significant difference (t=11.336, 7.983, 7.748, and 7.748, all P＜0.05).Conclusion The abnormal expression of miR-17 in gastric cancer tissues with Hp infection is significantly related to tumor diameter, TNM staging, Borrmann typing, lymph node metastasis, and prognosis, which can be used as a candidate marker for early diagnosis and prognosis of gastric cancer.
Keywords：gastric carcinomaHelicobacter pylorimicroRNA-375cytotoxin-related gene A
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 25-28 )