Feasibility of phage-display technology in screening specific polypeptide sequences in gastric cancer cells with high expression of CD44
LIN Xiaobo
JIA Huan
YU Zhaoxiang
LI Siguang
LI Zhen
WANG Wutao
Abstract：Objective To investigate the feasibility of phage-display technology in screening the specific polypeptide sequences in the gastric cancer cells with high expression of CD 44.Methods A phage-displayed random twelve-peptide library was used to screen CD44 purified protein for four rounds of affinity screening.Thirty clones were selected and were sequenced based on the single-strained DNA.ELISA was used to test the binding affinity of the selected phages to CD 44. The affinity of positive clones to CD44 integrated to the cell membrane was detected by immuno uorescence assay. Results During the four rounds, 30 clones were selected from the eluate of the 4th round of sequencing, and 7 sequences were determined.A six peptide motif which repeated 20 times was found in following analysis.Among the 7 sequences, 4 positive clones (S1, S2, S5 and S7) were identified by ELISA.The phage inserted sequence S1-WHXXXXXXQQA was identified, which had the highest affinity and specificity to CD 44.Conclusion Through phage-display technology, S1-WHXXXXXXQQA peptide sequence has the best affinity and specificity to CD 44, which may be a novel molecular probe for the molecular imaging diagnosis of gastric cancer.
Keywords：gastric carcinomaphagephage display technologypolypeptideCD44
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 10-13 )