Feasibility of using MNNG and CDCA to construct intestinal metaplasia model of gastric epithelial cells GES-1
LIU Hong
WANG Kun
JIANG Kailin
HUANG Yuancheng
LI Peiwu
GAO Shujing
PAN Jinglin
HUANG Deyu
LIU Fengbin
Abstract：Objective To investigate the feasibility of using N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) and goose deoxycholic acid (CDCA) to construct intestinal metaplasia (IM) model of gastric epithelial cells GES-1.Methods GES-1 cells were cultured in vitro, and then were treated with different concentrations of CDCA and MNNG for 24 h, and the values of OD at 450 nm wavelength were detected by CCK-8.The results showed that CDCA and MNNG had a growth inhibitory role on GES-1 cells, with 10%inhibition concentration (IC10) of 0.14 and 51.89 μmol/L, respectively.GES-1 cells were ran-domly divided into the CDCA group and MNNG group , and then were treated with CDCA and MNNG of IC 10, respectively.At the same time, GES-1 cells without medicine were taken as the control group.At 24 h, the expression of MUC1, MUC2, and CDX2 mRNA were detected by RT-PCR.Results Compared with the control group , the relative expression of MUC1 mRNA in the CDCA group and MNNG group decreased , the relative expression of MUC2 mRNA in the MNNG group decreased , and significant difference was found between every two groups (all P＜0.05).Conclusions CDCA and MNNG can inhibit the growth of gastric mucosa epithelial cells.Therefore, both CDCA and MNNG can be used to build IM models of gastric muco-sal cells.
Keywords：precancerous lesions of gastric carcinomaatrophic gastritisgastric mucosa epithelial cellsN-methyl-N′-nitro-N-nitrosoguanidinegoose deoxycholic acid
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )