Effects Of HIV-1 infection on related genes and proteins of ITAMs / ITIMs signaling pathway in human CD4 + T lymphoblast cells
MO Yuxiao
XIE Yanzheng
LIU Jing
GUO Xiaoqiang
HE Jinyang
Abstract：Objective To investigate the expression of related genes and proteins of immunoreceptortyrosine-based ac-tivation motifs(ITAMs)/ immunoreceptortyrosine-based inhibition motifs (ITIMs)signaling pathway in human CD4 + T lym-phoblast cells (M8166 cells)after HIV-1 infection. Methods M8166 cells cultured in vitro were randomly divided into the blank group and infection group. The cells in the blank group were normally cultured without special treatment,while the cells in the infection group were infected with 10 TCID50 HIV-1. The mRNA expression levels of related genes of ITAMs/ ITIMs signaling pathway were detected by real-time fluorescent quantitative PCR,and the expression of the protein of related receptors was detected by Western blotting. Results Compared with the blank group,the expression of SHP-1 mRNA in the M8166 cells increased after HIV-1 infection (P < 0. 01),and the expression of SHIP-1 mRNA showed a de-creasing trend but there was no statistical significance (P > 0. 05). No significant difference was found in the mRNA ex-pression of SHP-2,SHIP2,Zap70,and Syk (all P > 0. 05). On the other hand,the expression of SHP-1 and P-SHP-1 protein significantly increased after HIV-1 infection (both P < 0. 05),and the expression of SHIP1 and P-SHIP1 proteins significantly decreased (both P < 0. 05). The expression of Syk was slightly up-regulated and P-Syk was down-regulated, but there was no statistical difference (both P > 0. 05). No significant difference was found in the expression of SHP-2, SHIP2 and P-SHIP2 between these two groups (all P > 0. 05),and no expression was found in P-SHP2,Zap70,and P-Zap70. Conclusion The ITAMs/ ITIMs signaling pathway of M8166 cells is activated after HIV-1 infection.
Keywords：acquired immunodeficiency syndromehuman immunodeficiency virus-1human CD4 + T lymphoblast cellsM8166 cellsimmunoreceptortyrosine-based activation motifsimmunoreceptortyrosine-based inhibition motifsim-mune regulatory pathway
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )