Effect of overexpressed leukemia inhibitory factor receptor on apoptosisof lung cancer stem cells
JIANG Qi
REN Xuesong
LIU Chunhua
ZHANG Yan
TANG Qin
Abstract：Objective To explore the effect and mechanism of overexpressed leukemia inhibitory factor receptor ( LI-FR) on the apoptosis of lung cancer stem cells (LCSCs).Methods The primary lung cancer cells were isolated and cul-tured from primary cancer focus of patients with lung cancer .The CD133 +phenotype of LCSCs was selected by flow cytom-etry.The recombinant lentiviral overexpression plasmid containing LIFR and its negative control empty vector were used to infect LCSCs at the ratio of virus/cell number=18, and they were screened by 2 micron g/mL of purinomycin.The LCSCs with stable expression of LIFR and their control cell lines were successfully constructed , which were taken as the observa-tion group and the control group , respectively.The relative expression of LIFR mRNA in the two groups was detected by re-al-time quantitative PCR .The apoptosis rate of two groups was detected by flow cytometry at 48 h.The apoptosis-related proteins Bax, Bcl-2, and Caspase-3 in the two groups were detected by Western blotting .Results The expression levels of LIFR mRNA in the observation group and control group were 8.54 ±1.32 and 1.03 ±0.02, and the apoptosis rates were 35.00％±5.29％and 11.33％±1.76％, respectively (all P<0.05).Compared with the control group , the expression of Bcl-2 protein in the observation group decreased , and the expression of Bax and Caspase-3 protein increased ( all P<0.05).Conclusion Overexpression of LIFR can promote the apoptosis of LCSCs by inhibiting the expression of Bcl -2 pro-tein and promoting the expression of Bax and Caspase-3 protein.
Keywords：leukaemia inhibitory factor receptorlung cancer stem cellsapoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )