Osteoinductivity of icariin on MC3T3-E1 cells
WANG Jianru
ZHANG Yumin
HAN Bo
Abstract：Objective To evaluate cytotoxicity and osteoinductivity of icarrin on MC 3T3-E1 osteoblast precursor cells, which provides basis for bone tissue regeneration.Methods The MC3T3-E1 cells were randomly divided into the observa-tion group,the negative control group,and the positive group.The cells in the observation group were cultured with 100μL of 1×10-6,1×10-5,1×10-4,1×10-3,and 1×10-2mmol/L icarrin,respectively;cells in the negative control group were added with the same volume of DMSO and cells in the positive group with the same volume of rhBMP-2.Subsequently,the proliferation rate on the 3rd and 7th day was tested with MTT method.The cell survival rate was determined by dead/living cell fluorescence detection.The calcium nodules were observed by Alizarin red calcium nodules stain.The calcium con-tent,alkaline phosphatase(ALP),and osteocalcin were tested by cellular ultrasonic cracking.Results The cell prolifer-ation rate in the observation group with 1×10-2mmol/L icariin was lower than that in the positive and negative groups on the 3rd day(both P<0.05), and the cell proliferation rate in the observation group with 1×10-2mmol/L and 1×10-3 mmol/L icariin was lower than that in the positive and negative groups on the 7th day(both P<0.05).The cell survival rate in the observation group with 1×10-3mmol/L icariin was lower than that in the positive group(P<0.05).The cell survival rate in the observation group with 1×10-2mmol/L icariin was lower than that in the positive and negative groups (both P<0.05).No calcium nodules formed in the negative group and a large area of calcium nodules was found in the positive group.In the observation group,the calcium nodule gradually increased with the increasing concentrations of icari -in.The ALP activity and osteocalcin in the observation group were higher than those in the negative group(all P<0.05). The Ca content in each of the observation group with 1×10-4,1×10-3,and 1×10-2mmol/L icariin was higher than that in the negative group,and the Ca content in each of the observation group with 1×10-6,and 1×10-5mmol/L icariin was low-er than that of the positive group(all P<0.05).Conclusion 10-4mmol/L icariin, with lower cytotoxicity and osteoin-ductivity,which is similar to rhBMP-2,can be used as an alternative agent of growth factor combined with bone repair ma-terials to promote the bone tissue regeneration.
Keywords：icariinosteblast precursor cellcytotoxicitybone defectosteoinductivitybone repair materials
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )