Regulation effect of p-mTOR/p70S6K signaling pathway in inflammation of acute necrotizing pancreatitis

HUANG Yangfeng; JIANG Wei

doi:10.3969/j.issn.1002-266X.2018.13.005

Abstract：Objeetive To investigate the regulation effect of phosphorylated mammalian target of rapamycin (p mTOR)/p70 ribosomal protein S6 kinase(p70S6K) signaling pathway in the inflammatory response of acute necrotizing pancreatitis (ANP).Methods Ninety-six male SPF rats were randomly divided into the ANP model group,rapamycin group,and blank control group.The rats in the ANP model group were injected with 1 mL/kg of 5 ％ sodium taurocholate in the opposite direction of pancreatic duct.The rats in the rapamycin group were injected with 0.5 mg/kg rapamycin intraperitoneally on the basis of the model group.Serum samples were collected from 8 rats in each group at 3,6,12,and 24 h after the model was established,and the levels of AMY,TNF-α,IL-6,and IL-10 in the serum were measured.Pancreatic tissues were taken for the detection of p-mTOR and p70S6K protein expression by Western blotting,for histopathological observation by HE staining,and apoptotic index by TUNEL.Results At each time point,the serum levels of AMY,TNF-α,and IL-6 in the rapamycin group were significantly lower than those in the ANP group (all P ＜ 0.01),but IL-10 was significantly higher than that of the ANP group (P ＜ 0.01).All the parameters in the ANP model group and rapamycin group were higher than those of the blank control group (all P ＜ 0.05).The expression of p-mTOR and p70S6K in the rapamycin group was significantly lower than that in the ANP model group (both P ＜0.01).The apoptosis index of the rapamycin group was also significantly lower than that of model group (P ＜ 0.01).Histopathological examination showed that the degree of lesions in the pancreatic tissues of the rapamycin group was lighter than that in the model group.Conclusion Decreased activity of p-mTOR/p70S6K pathway can improve inflammation and reduce pancreatic tissue lesions in ANP rats.