Effects of FOXQ1-siRNA on invasion and migration of papillary thyroid carcinoma TPC-1 cells

ZHONG Ye; YANG Guanghua; XIAO Tong; WANG Xiaotao; TIAN Wei; ZHANG Guozhi

doi:10.3969/j.issn.1002-266X.2018.13.003

Abstract：Objective To investigate the effects of FOXQ1-siRNA on the invasion and migration of papillary thyroid carcinoma TPC-1 cells and its related mechanisms.Methods The FOXQ1-siRNA and non-sense sequence siRNA were transfected into the TPC-1 cells as the experimental and nonsense sequence group by lipofectamineTM2000,and the blank group did not receive any treatment.The expression of FOXQ1 mRNA and protein was detected by qRT-PCR and Western blotting,respectively.The expression of phosphorylated protein kinase B (p-AKT),matrix metalloproteinase 7,9 (MMP-7 and MMP-9) in the TPC-1 cells was detected by Western blotting.At 48 h,the invasion and migration of cells were observed by Transwell chamber assay.Results The relative expression levels of FOXQ1 mRNA and protein in the experimental group were significantly lower than those in the blank group and the nonsense sequence group,and the difference was statistically significant (all P ＜ 0.05).The relative expression levels of p-AKT,MMP-7,and MMP-9 in the experimental group were significantly lower than those in the blank group and the nonsense sequence group,and the difference was statistically significant (all P ＜ 0.05).At 48 h after transfection,the number of cells passing through the cell membrane in the experimental group was significantly less than that in the blank group and the nonsense sequence group,and significant difference was found (all P ＜ 0.05).Conclusion FOXQ1-siRNA inhibits the invasion and metastasis of TPC-1 cells by inhibiting the PI3K/AKT signaling pathway.