Amplification and bioinformatics analysis of a specific EST fragment of bladder cancer tissues
ZHAO Zhenli
HU Shaohua
HAO Chuan
SHI Shu
REN Laicheng
Abstract：Objective To amplify the total length of a specific expressed sequence tag(EST)fragment of bladder cancer and to perform the bioinformatics analysis on it.Methods We collected 1 case of bladder cancer tissues which were pathologically confirmed bladder transitional cell carcinoma.The RNA from the bladder cancer tissues was extracted, and then we obtained the 5′end DNA sequence and 3' end DNA sequence of the EST fragment by RACE,and used the GeneTool to synthesize the results of DNA sequence.We analyzed the full length sequence of cDNA and its corresponding protein with bioinformatics,and submitted it to the NCBI to get GenBank accession number and protein ID by sequin soft -ware.Results We successfully got the 5′end and 3′end of the specific EST fragment,and used the GeneTool to synthe-size a full sequence of 1839bp,and it had integrated ORF,chromosome was located in 17p13.3.We got the GenBank ac-cession number,that was KR780251 in NCBI,and the ID of polypeptide sequence acquired protein translationed by ORF was AKO62675.Conclusion The gene of bladder cancer tissue-specific EST fragment is KR780251 with a full-length se-quence of 1839 bp and contains an intact ORF.The encoded protein AKO62675 may be a mutant from the normal bladder tissues.
Keywords：urinary bladder carcinomageneexpressed sequence tagmutationKR780251AKO62675bioinfor-matics
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 18-21 )