Effects of gypenosides on cell morphology and migration ability of human glioblastoma cell line U87
WEI Yilan
QU Jie
DOU Zhijie
SHEN Xiaoping
Abstract：Objective To observe the effects of different doses of gypenosides (Gyp) on the migration and cell morphology of human glioblastoma U87 cells in vitro and to explore the mechanism.Methods U87 cells in the logarithnic growth phase were added with 100 μL of 10 and 5 mg/mL Gyp solution and the same volume of PBS (referred as groups A,B,and C),and then given the routine cultivation.The cell morphology,migration ability,and interleukin-6 protein levels were compared in each group.Results In the group A,the cells grew very slowly with small volume,the pyknosis was very obvious and the phenomenon of cell attachment disappeared nearly.In the group B,the cells grew slowly with reduced volume and cytoplasm pyknosis,some cells even ruptured and died,and the cell attachment decreased.In the group C,the cell growth was regular with clear outline and full-shape,the cell body was translucent,and the adherent growth was good.The area of cell migration in the groups A,B,and C accounted for 54.8％,82.4％,and 98.6％ of the original scratch area (all P＜0.05);the number of transmembrane cells in the groups A,B,and C accounted for 19.8％,58.9％,and 100％ (all P ＜ 0.05);the protein levels of IL-6 in the groups A,B,and C were high,moderate,and low,respectively;significant differences were found between every two groups (all P ＜ 0.05).Conclusion The Gyp inhibits the growth and metastasis ability of U87 cells with the best effect at 10 mg/mL Gyp,and the mechanism may be associated with the inhibition of IL-6 protein in U87 cells.
Keywords：gypenosidesglioblastomaU87 cellscell morphologycell migrationinterleukin 6 protein
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )