Effects of KU55933 on proliferation and apoptosis of glioblastoma cells U87 after chemotherapy
WANG Qian
CAI Weisong
Abstract：Objective To explore the influence and mechanism of inhibition of ATM/ATR(KU55933) on the proliferation and apoptosis of glioblastoma cells U87 treated with chemotherapy.Methods U87 cells were randomly divided into three groups:KU55933 group,TMZ group,and control group (without intervention).In the KU55933 group,10 μmol/L KU55933 was added,and after 1 h,50 mg/mL temozolomide (TMZ) was added;in the TMZ group,50 mg/mL TMZ was added.The cells were cultured in the 37 ℃ and 5％C02 incubator.Then we compared the OD values,apoptosis rates,and relative protein expression of cell cycle checkpoint kinase 1 (Chk1),phosphorylation Chk1 (pChk1),cell cycle checkpoint kinase 2 (Chk2),phosphorylation Chk2 (pChk2),Cyclin B,Cyclin D1,and Cleave-Caspase-3.Results The TMZ group and the KU55933 group had significantly lower OD values at 24,48,and 72 h as compared with the control group (all P ＜ 0.05),and the KU55933 group had significantly lower OD values at 24,48,and 72 h as compared with the TMZ group (all P ＜ 0.05).The TMZ group and the KU55933 group had significantly higher apoptosis rates as compared with the control group (all P ＜ 0.05),and the KU55933 group had a significantly higher apoptosis rate as compared with the TMZ group (P ＜ 0.05).The relative protein expression levels of pChk1,pChk2,and Cleaav-Caspase-3 in the TMZ group and the KU55933 group were significantly higher than those in the control group (all P ＜ 0.05),and the relative protein expression levels of Cyclin B and Cyclin D1 in the TMZ group and the KU55933 group were significantly higher than those in the control group (both P ＜ 0.05);compared with the TMZ group,the KU55933 group had significantly lower relative protein expression of pChk1,pChk2,Cyclin B,Cyclin D1 and a higher relative protein expression of Cleaav-Caspase-3.Conclusion KU55933 may significantly inhibit the proliferation and accelerate the apoptosis of U87 cells after chemotherapy by down-regulating the expression of pChk protein,Cyclin B protein,Cyclin D1 protein,and reversing the cell cycle arrest.
Keywords：inhibition of ATM/ATRtemozolomideglioblastoma
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )