Effect of hsa-miR-202 on biological behavior of cisplatin-resistant cell in non-small-cell lung cancer

PAN Lei; YIN Jun; FU Wenfan; DAI Lu; ZHANG Feng; ZHAO Jian

doi:10.3969/j.issn.1002-266X.2018.08.005

Abstract：Objective To investigate the effect of hsa-miR-202 on the biological behavior of cisplatin-resistant A549/ DDP cells (hereinafter referred to as A549/DDP cells) of non-small-cell lung cancer (NSCLC). Methods A549/DDP cells were subcultured and the cells of passage 3 in the logarithmic growth phase were inoculated on 6-well plate and randomly divided into the blank control group, negative control group, and hsa-miR-202 group. Cells in the negative control group and hsa-miR-202 group were transfected with mimic NC and hsa-miR-202 mimic, respectively, and the blank control group was not transfected. The cells were collected at 24 h after transfection and the proliferation rate of the cells was detected by MTT assay at 24, 48, 72, 96 and 120 h after transfection. The number of colony formation was measured by plate colony formation assay. The cell cycle was detected by flow cytometry, and the apoptosis rate was detected by FITC Annexin V/PI double staining. Results The cell proliferation rate in the hsa-miR-202 group was significantly lower than that in the blank control group and negative control group (P＜0. 05); the number of colony forming in the hsa-miR-202 group was significantly lower than that in the blank control group and negative control group (P＜0. 05); the proportion of cells in the G1 phase of the hsa-miR-202 group was significantly higher than that in blank control group and negative control group, and the proportion of cells in S phase and G2 phase was significantly lower than that in the blank control group and negative control group; the apoptosis rate of the hsa-miR-202 group was significantly higher than that of the blank control group and the negative control group (P < 0. 05); no significant differences were found in the above parameters between the blank control group and negative control group (all P > 0. 05). Conclusion Overexpression of hsa-miR-202 can inhibit the pro-liferation of A 549/DDP cells and promote their apoptosis.