Expression of miR-381 and its effect on proliferation,migration, and invasion of renal carcinoma cells
LIANG Yingying
MO Zhiwen
HUANG Yufan
SHAO Xunfan
YUAN Yawei
Abstract：Objective To observe the expression changes of miR-381 in the renal cell carcinoma cell line, and its effect on the proliferation,migration,and invasion of renal cell carcinoma cells.Methods The expression of miR-381 in three renal cell carcinoma cell lines(Caki-2,A498,and 786-O)and normal kidney cell line HK-2 was detected by fluo-rescent quantitative PCR.The Caki-2 cell line was divided into three groups,miR-381 group which was transfect with miR-381 mimics,mock group which ws transfected with miR-381,and the NC group without transfection.CCK-8,Scratch test, and Transwell assay were used to determine the proliferation,migration,and invasion capabilities.The real-time PCR and Western blotting were used to detect the mRNA and protein levels of ID-1 in these three groups.Results The expression level of miR-381 in the Caki-2,A498 and 786-O cells was significantly lower than that in the HK-2 cells(P<0.01).At 72 and 96 h after transfection,the proliferation of miR-381 group was lower than that of the NC group and mock group(P<0.01).The wound healing rate was 28.7 ±2.6%in the miR-381 group,56.4 ±4.5% in the NC group, and 59.7 ± 4.8%in the mock group, and the wound healing rate of the miR-381 group was significantly lower than that in the NC group and mock group(all P<0.01).The invasive cells were 44.7 ±3.9 in the miR-381 group,225.7 ±15.7 in the NC group,and 235.9 ±17.9 in the mock group,and the invasive cells were significantly less than those of the NC group and mock group(all P<0.01).Compared with the NC and mock groups, the mRNA and protein expression of ID-1 in the cells with over-expression of miR-381 was lower(P<0.01).Conclusions The miR-381 is low expressed in the renal cell carcinoma cells.Over-expression of miR-381 can inhibit the proliferation,migration,and invasion of renal cell carci-noma cells,which may be associated with the down-regulated expression of ID-1.
Keywords：renal cell carcinomamicroRNA-381ID-1cell proliferationcell migrationcell invasion
Publication Date：2018-01-01
Pages：4( 1-4 )