Reverse effect of Ejiao (colla corii asini) on imbalance of Th17/Treg subsets of mice with airway inflammation
ZHANG Zhe
MA Yun
HU Jinghong
LAN Hongyun
ZHANG Yongqing
YAO Chengfang
Abstract：Objective To investigate the therapeutic effect of Ejiao (colla corii asini) on the imbalance of Th1T/Treg subsets in the lung tissues of mice with smoking-induced airway inflammation.Methods Twenty-four male C57BL/6 mice were randomly divided into the control group,model group,and Ejiao (colla corii asini) group (Ejiao group).Mice in the model group and Ejiao group were exposed to cigarette smoking.Mice in the model groupwere administered 0.2 mL PBS daily,mice in the Ejiao group were administered 0.2 mL Ejiao (colla corii asini) solution (0.2g/ml) daily,and mice in the control group were fed normally.Twenty-four weeks later,the histopathological condition of lung in mice of each group was observed.The proportion of helper T cell 17 subsets (Th17) and regulatory T cell subsets (Treg) in the lung tissues were detected by flow cytometry.The expression of IL-6,IL-17A,and Foxp3 mRNA in the lung tissues was detected by reverse transcription polymerase chain reaction (RT-PCR),and the serum levels of IL-6,IL-17A,and Foxp3 were detected by enzyme-linked immunosorbent assay.Results Compared with the control group,the lung tissues of mice in the model group were infiltrated with large number of inflammatory cells,alveoli ruptured and fused,the proportion of Th17 cells and Treg cells in the lung tissue increased,and the levels of IL-17A,IL-6,Foxp3 in the serum,and IL-17A,Foxp3,and IL-6 mRNA in the lung tissues increased (all P ＜ 0.05).Compared with the model group,the inflammation of the lung tissues of mice in the Ejiao group was significantly improved,the proportion of Th17 cells and Treg cells in the lung tissues decreased,and the levels of serum IL-17A,IL-6,and Foxp3,and IL-17A,Foxp3,and IL-mRNA in the lung tissues decreased (all P ＜ 0.05).Conclusions Smoke can induce the increased proportion of Th17 and Treg subsets and up-regulate the expression of IL-6,IL-17A,and Foxp3 cytokines in the lung tissues of mice with airway inflammation.Ejiao (colla corii asini) can reduce the lung inflammation in mice by reducing the proportion of Thl7 and Treg subsets and expression of IL-6 and IL-17A,and Foxp3.
Keywords：cigarette smoking exposureEjiao (colla corii asini)airway inflammationhelper T cell 17 subsetsregulatory T cells
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 11-14 )