Effects of total flavonoids of semen litchi on TGF-β1-induced apoptosis of human hepatic stellate cells
CAO Jie
LIN Lixin
QIN Guijin
XIAO Xuhua
HUO Qun
ZHAO Yongzhong
Abstract：Objective To investigate the effects of total flavonoids of semen litchi (TFL) on the apoptosis of human hepatic stellate cells LX2(HSC-LX2) induced by transforming growth factor β1 (TGF-β1),and to explore the related molecular mechanisms.Methods The cultured HSC-LX2 cells were randomly divided into the normal group,TGF-β1 group,and the different concentrations of TFL groups (150,300,and 600 mg/L TFL).Cells in the TGF-β1 group and different concentrations of TFL groups (150,300,and 600 mg/L TFL) were cultured in the culture dish with DMEM (10％ FBS) and TGF-β1 (5 μg/L);after 24-hour culture,different concentrations of TFL groups (150,300,and 600 mg/L TFL) were added with different concentrations of TFL (150,300,and 600 mg/L TFL);after 48 h,the related indicators were measured.The morphological changes of cells were observed by Hoechst33258 staining;the cell cycle was detected by flow cytometry;FITC Annexin V/PI double staining was used to detect the apoptosis;the expression levels of TLR4,NF-κB,and IL-1R Ⅰ proteins were detected by Western blotting.Results The nuclear morphology of cells in the normal group and the TGF-β1 group was complete,the proliferation was active in the TGF-β1 group,and the morphological changes of apoptosis including pyknosis,lysis and apoptotic bodies of nucleus were observed in the different concentrations of TFL groups (150,300,and 600 mg/L TFL).Flow cytometry showed that with the increasing TFL concentrations,the cells in the G0/G1 phase increased in the TFL groups (P ＜ 0.05),decreased in the S phase (P ＜ 0.05);with the increasing TFL concentrations,TFL promoted the apoptosis of HSC-LX2,and tended to promote late apoptotic.In the different concentrations of TFL groups (150,300,and 600 mg/L TFL),TLR4,NF-κB,and IL-1R Ⅰ protein expression was lower than that of the TGF-β1 group (P ＜0.05);with the increase of TFL concentrations,the expression of TLR4,NF-κB,and IL-1R Ⅰprotein in HSC-LX2 decreased gradually (all P ＜ 0.05).Conclusion TFL can promote the TGF-31-induced apoptosis of HSC-LX2 cells,especially for the late apoptosis,by reducing the expression of TLR4,NF-κB,and IL-1R Ⅰ.
Keywords：hepatic fibrosistotal flavonoids of semen litchihuman-hepatic stellate cellsapoptosistransforming growth factor β1
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )