Effects of HSP70 siRNA on collagen expression in the human keloid fibroblasts
WANG Yangyang
LI Xiaojing
Abstract：Objective To investigate the effects of heat shock protein 70(HSP70)small interfering RNA(HSP70 siRNA)on the collagen production of the human keloid fibroblasts.Methods Collecting 56 cases of human keloid tis-sues,isolating the human keloid fibroblasts and culturing them in vitro.The human keloid fibroblasts were divided into the observation group,the negative group and the blank control group.The HSP70 siRNA and the negative siRNA were trans-fected into the cells of the observation group and the negative group by the liposome, respectively.Moreover, the blank control group was not transfected.At 24 h after the transfection,the mRNA expression levels of HSP70,collagen Ⅰ,and collagen Ⅲwas detected by the real-time PCR.The protein expression levels of HSP70,collagen I,and collagen Ⅲwere detected by Western blotting.The total collagen in the medium was analyzed by the Sircol collagen assay.Results Com-pared with the negative group and the blank control group,the mRNA and protein expression levels of HSP 70,collagenⅠ, and collagen Ⅲdecreased in the observation group,as well as the total collagen in the medium(all P<0.05).However, there were no differences between the negative group and the blank control group(all P>0.05).Conclusion Transfect-ing the HSP70 siRNA could inhibit the collagen expression in the human keloid fibroblasts.
Keywords：heat shock protein 70RNA interferencesiRNAhuman keloid fibroblastscollagen
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-20 )