Influence of carboxymethylated ganoderma lucidum polysaccharide pretreatment on cerebral ischemia-reperfusion injury in rats

LI Yawei; CHEN Lihong; HAN Liqin; ZHOU Dapu; LIU Pan; JIN Ying; ZHU Wenhe

doi:10.3969/j.issn.1002-266X.2018.04.004

Abstract：Objective To observe the influence of carboxymethylated ganoderma lucidum polysaccharide(CM-GLP) pretreatment on the cerebral ischemia-reperfusion injury in rats and its possible mechanism.Methods Totally 100 SD rats were randomly divided into five groups:the sham group,the model group,the GLP group,the CM-GLP group,and the ni-modipine group,with 20 in each.The GLP group received intraperitoneal injection of GLP 40 mg/(kg· d),the CM-GLP group received CM-GLP of 40 mg/(kg· d),the nimodipine group received nimodiping of 1 mg/(kg· d), and the sham group and the model group both received the same volume of normal saline.The rat cerebral ischemia reperfusion model was established by the middle cerebral artery occlusion method in rats of all groups except for the sham group.At 24 h after is-chemia-reperfusion,the neurologic deficit score was detected as well as the content of water in brain tissues.The expression of superoxide dismutase(SOD)and malondialdehyde(MDA),the levels of NF-κB,TNF-α,IL-1,and IL-6 in the brain tissues were detected by ELISA.Western blotting was used to detect the protein expression levels of heat shock protein -70 (HSP-70)and p-Akt.Results The neurologic deficit score of the model group was significantly higher than that of the sham group,and the GLP group,the CM-GLP group,and the nimodipine group were lower than the model group,further-more,the CM-GLP group and the nimodipine group decreased more significantly(P<0.01).The content of water in the brain tissues of the rats,the levels of MDA and the levels of NF-κB,TNF-α,IL-1,and IL-6 of the model group were high-er than those of the sham group,while the SOD activity and the protein expression of p-Akt and the HSP-70 were lower than those of the sham group(all P<0.01).The content of water in the brain tissues of the rats,the levels of MDA and the levels of NF-κB,TNF-α,IL-1,and IL-6 of the GLP group,the CM-GLP group,and the nimodipine group were lower than those of the model group,while the SOD activity,the protein expression of p-Akt,and the HSP-70 were higher than those of the mod-el group,furthermore the CM-GLP group and the nimodipine group changed more significantly(P<0.05 or P<0.01).Con-clusion CM-GLP pretreatment can effectively relieve the cerebral ischemia-reperfusion injury in rats by regulating the HSP70/PI3K/Akt signaling pathway and inhibiting the inflammatory reaction damage to the nerve cells after reperfusion.