Expression changes of TLR4/MyD88/NF-κB signaling pathway in target organs of mice with acute graft-versus-host disease
JIANG Yangyang
TU Sanfang
WU Yuanbin
LI Yuhua
Abstract：Objective To investigate the expression changes of Toll-like receptor-4(TLR4)/myeloid differentiation primary response gene 88(MyD88)/nuclear factor-κB(NF-κB)signaling pathway protein in mice with acute graft-versus-host disease(aGVHD)after allogeneic bone marrow transplantation(allo-BMT).Methods Allogeneic bone marrow trans-plantation was performed with C57BL/6 male mice as donors and BALB/C female mice as recipients.The aGVHD models of mice(aGVHD group)were established by injecting the donor bone marrow cells and splenic lymphocytes after the recipients were treated with the lethal dose preconditioning regimen of Busulfan +cyclophosphamide.Syngeneic bone marrow transplan-tation(Syn-BMT)was performed with BALB/C male mice as donors and BALB/C female mice as recipients.The bone mar-row cells from donor mice were injected into the recipient mice after equal dose of preconditioning regimen,and the syngeneic bone marrow transplantation model was established as the control group.On the 14 day after transplantation,5 survival mice were taken from the aGVHD group and the control group,respectively.The liver, intestine, spleen, and skin tissues were taken from the mice.Real-time quantitative polymerase chain reaction(qRT-PCR), Western blotting, and immunohisto-chemistry were used to detect the mRNA and protein expression rates of TLR 4,MyD88,NF-κB p65 and p50 in the target or-gans of mice.Results The mRNA expression and protein expression,and the the protein expression rates of TLR4,MyD88, NF-κB p65 and p50 in the liver,intestine,spleen,and skin tissues of the GVHD group were higher than those of Syn-BMT control group(all P<0.05).Conclusion The expression of TLR4/MyD88/NF-κB signaling pathway protein in the liver, intestine,spleen and skin of aGVHD model mice is up-regulated at the gene level and protein level, suggesting that the TLR4/MyD88/NF-κB signaling pathway may be involved in the pathogenesis of aGVHD after allo-BMT.
Keywords：acute graft-versus-host diseaseallogeneic bone marrow transplantationToll-like receptor-4myeloid differentiation primary response gene 88nuclear factor-κB
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 8-11 )