Effect of placenta-derived mesenchymal stem cells modified by HLA-G on proliferation of CD4 +T lymphocytes
CUI Guiyu
BAI Jian
MIAO Lanying
LIN Dayong
Abstract：Objective To investigate the effect of placenta-derived mesenchymal stem cells modified by human leuko-cyte antigen G(HLA-G)on the proliferation of CD4 +T lymphocytes.Methods Placenta-derived mesenchymal stem cells from newborn were cultured and identified.Placenta mesenchymal stem cells in logarithmic phase were randomly divided into the PEGFP-N1-HLA-G group,PEGFP-N1 group,and the control group.The former two groups were separately trans-fected with PEGFP-N1-HLA-G plasmid and empty vector PEGFP-N1.The control group was cultured conventionally.After culturing for 20 h,HLA-G protein was detected.The results suggested that PEGFP-N1-HLA-G plasmid promoted the ex-pression of HLA-G protein,and the transfection was successful.In vitro, CD4 +T lymphocytes were isolated from healthy people peripheral blood and mixed with the cells in each group.After culturing for 24,48 and 72 h,the inhibition rate of CD4 +T lymphocyte growth was detected by MTT assay,respectively.Results The proliferation inhibition rate of CD4 +T lymphocytes in the PEGFP-N1-HLA-G group was higher than that in the control group and PEGFP-N1 group at 24,48,and 72 h after co-culture(all P<0.05).Conclusion The placental mesenchymal stem cell modified by HLA-G gene can in-hibit the proliferation of CD4 +T lymphocytes.
Keywords：placental mesenchymal stem cellshuman leukocyte antigen GT lymphocytescell proliferation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )