Effects of leukaemia inhibitory factor receptor on lung metastasis of cervical cancer
HE Dan
LIU Haifeng
JIANG Xinni
Abstract：Objective To investigate the role of leukemia inhibitory factor receptor(LIFR)and Hippo-YAP signaling pathway in the lung metastasis of cervical cancer.Methods ① The real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of LIFR mRNA in the primary cancerous tissues,adjacent non-can-cerous tissues and metastasis tissues from 50 cases of patients with cervical cancer and lung metastasis.② Recombinant lentiviral plasmid containing LIFR or its negative control(vector)were used to infect the human cervical cancer cell line SiHa.After screening with 2.0 μg/mL puromycin, the cells that stably expressed LIFR(observation group)or vector (control group)were obtained.Western blotting was used to detect the expression of LIFR and Hippo-YAP signaling path-way related proteins YAP, P-YAP, TAZ, and p-TAZ.The cells of the two groups were injected into the BALB /c nude mice through the tail vein.After 4 weeks,the nude mice were killed and the lung tissue was removed for HE staining.The number of all the metastases in the lung tissues was counted microscopically.Results ①The relative expression of LIFR mRNA in the primary tumor was higher than that in the adjacent non-cancerous tissues,and the relative expression of LIFR mRNA in the metastasis tissues was lower than that in primary tumor and adjacent non -cancerous tissues(all P<0.05).②The relative expression of LIFR,p-YAP and p-TAZ proteins in the SiHa cells which overexpressed LIFR was higher than that in the control cells(all P<0.05).There was no significant difference in YAP and TAZ protein expression between the control group and observation group(all P>0.05).The lung metastases in the SiHa cells with overexpressed LIFR and control cells were 9 ±2 and 60 ±5, respectively(all P<0.05).Conclusion Overexpression of LIFR can promote the phosphorylation of Hippo-YAP signaling pathway proteins YAP and TAZ,and then inhibit the formation of lung metastasis of cervical cancer cells.
Keywords：cervical carcinomalung metastasisleukaemia inhibitory factor receptorleukaemia inhibitory factorHippo-YAP
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )