Screening and functional identification of peptides specifically binding to Paclitaxel-resistant cervical cancer cells Hela
LI Qian
ZUO Xuan
DENG Chengcheng
YIN Dongfeng
Abstract：Objective To screen out the peptides specifically binding to Paclitaxel-resistant cervical cancer cells Hela (Hela/PTX).Methods Using drug-resistant cervical cancer Hela/PTX cells as the target cells,and the phage 7 peptide library was screened for 3 rounds by competitive binding tests.Using ELISA to determine the affinity of peptides to Hela/PTX cells(P/N value).Ten peptides with the high P/N value were selected to be sequenced,and we named a polypeptide with the highest P/N value and synthesized this peptide in vitro according to its sequence.The molecular weight of the pep-tide was determined by mass spectrometry.The affinity of the peptide to Hela/PTX and osteosarcoma cells was observed by cell affinity experiment.Results The selected peptide was named EP-7.Mass spectrometry analysis showed that the mo-lecular weight of EP-7 was 815.30D.The results of cell affinity test showed that there was green fluorescence in the cyto-plasm of Hela/PTX,but no green fluorescence was observed in osteosarcoma cells.Conclusion The peptide EP-7 which can bind specifically to Hela/PTX cells is successfully screened out.
Keywords：cervical carcinomadrug-resistant cervical cancer cellsphage peptide librarypolypeptide
Publication Date：2018-01-21
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 31-33 )