Effects of different doses of kaempferol on proliferation and apoptosis of pancreatic cancer PANC-1 cells
CHEN Zhuo
LIU Shengnan
WU Kejian
CHEN Guangxia
HE Xiaohua
Abstract：Objective To observe the proliferation,apoptosis,and cell cycle distribution of pancreatic cancer PANC-1 cells cultured with different doses of kaempferol, and to explore its mechanism.Methods PANC-1 cells cultured in vitro were divided into the control group and the observation groups 1,2,3,and 4.Cells in the observation groups 1,2, 3,and 4 were added with 20,40,80,and 160 μmol/L kaempferol,while cells in the control group were given the same volume of normal saline.CCK-8 was used to observe the cell proliferation,flow cytometry was used to measure the apopto-sis rate and observe the cell cycle distribution,and Western blotting was applied to detect the intracellular TLR-4,NF-κB, VEGF,and Caspase-3.Results ①With the same time of treatment, the proliferation inhibitory rate of PANC-1 cells gradually increased with the increase of kaempferol concentrations(all P <0.05); with the same concentration of kaempferol,the proliferation inhibitory rate of PANC-1 cells gradually increased with the increase of the treatment time(all P<0.05).②As the kaempferol concentration increased, the proportion of PANC-1 cells in the S phase gradually in-creased(all P<0.05),and the proportion of PANC-1 cells in the G0/G1phase decreased gradually(all P<0.05).③With the increase concentrations of kaempferol,the apoptosis rate of PANC-1 cells gradually increased(all P<0.05).④With the increased concentrations of kaempferol, the relative expression of TLR-4, NF-κB and VEGF in PANC-1 cells gradually decreased(all P <0.05).Conclusions The proliferation of pancreatic cancer PANC-1 cells cultured with kaempferol is inhibited,the apoptosis rate increases,and the proportion of cells in the S phase increases with a dose-de-pendent manner.This may be due to that the kaempferol culture leads to the decreased expression of TLR -4,NF-κB,and VEGF,and the increased expression of Caspase-3 in pancreatic cancer PANC-1 cells.
Keywords：kaempferolpancreatic carcinomacell proliferationapoptosiscell cycleTLR-4/NF-κB signaling pathway
Publication Date：2018-01-21
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 27-30 )