Single nucleotide polymorphisms of TNF-α gene promoter-308G/A in patients with ulcerative colitis
CHANG Tingmin
ZHANG Chaoxian
ZHANG Lili
LI Xiumin
Abstract：Objective To analyze the single nucleotide polymorphisms of tumor necrosis factor-α (TNF-α) gene promoter-308G/A in patients with ulcerative colitis (UC).Methods The single nucleotide polymorphisms of TNF-α gene promoter-308G/A were analyzed in the peripheral blood leukocytes of 750 patients with confirmed UC (observation group) and 750 healthy persons (control group) by polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP).The expression of TNF-α in plasma was determined by ELISA.Results The frequencies of GG,GA,AA genotypes and G,A allele in the observation group were 14.93％,42.27％,42.80％,36.07％,and 63.93％,respectively,and 67.73％,16.40％,15.87％,75.93,and 24.07 in the control group,all P ＜0.05.The levels of TNF-α in the plasma of the observation group and control group were (31.48 ± 11.52) pg/L and (14.82 ± 5.61) pg/L,respectively (P ＜0.05).The levels of TNF-α in patients with GG,GA and AA genotypes of the observation group were (17.95 ±7.82),(34.32 ± 0.75),and (34.64 ± 13.83) pg/L,respectively,while those in the control group were (10.63 ± 3.29) and (21.25 ± 10.47),and (21.91 ± 8.51) pg/L,respectively.The differences were significant between patients with GA and AA genotypes and patients with GG genotype in the same group,and between patients with GG,GA and AA genotypes of the two groups (all P ＜ 0.05).Conclusion There is single nucleotide polymorphism of TNF-α gene promoter-308G/A in patients with UC,and GA and AA genotypes are the risk factors for UC,because they can increase the TNF-α expression in plasma.
Keywords：tumor necrosis factor-α gene promoter-308G/Asingle nucleotide polymorphismulcerative colitistumor necrosis factor-α
Publication Date：2017-12-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-23 )