Effects of temozolomide on proliferation and apoptosis of human glioma cells U251

ZHANG Yongsen; YANG Limin; YANG Weilong; LYU Linya; WANG Yufeng

doi:10.3969/j.issn.1002-266X.2017.47.005

Abstract：Objective To observe the effects of temozolomide on the proliferation and apoptosis of human glioma cells U251 and to explore its possible mechanism.Methods U251 cells in the logarithmic growth phase were selected and then were added with 0.02、0.06、0.1 g/L temozolomide,but the cells in the blank control group were not given.These cells were cultured for 24,48 and 72 h,respectively.MTT was applied to observe the cell proliferation.U251 cells in the logarithmic growth phase were selected,and then were added with 0.06 g/L temozolomide (as the 0.06 g/L temozolomide group).After U251 cells were cultured for 48 h with different concentrations of temozolomide,flow cytometry was used to determine the apoptosis rate,and RT-PCR and Western blotting were used to detect the expression level of survivin mRNA.Results The cellular proliferation inhibition rate of U251 cells increased over the time and the increased concentrations of temozolomide.The apoptosis rates of the 0.06 g/L temozolomide group and the control group were 22.6％ ± 2.3％ and 3.3％ ± 1.1％,respectively;the relative expression of survivin mRNA was 0.65 ±0.11 in the 0.06 g/L temozolomide group,and was 0.96 ± 0.15 in the control group;the expression of survivin protein was 0.25 ± 0.03 in the 0.06 g/L temozolomide group,and was 0.49 ± 0.04 in the control group;the differences were significant between these two groups,all P＜ 0.05.Conclusion Temozolomide can effectively inhibit the proliferation of U251 cells and induce the apoptosis by inhibiting the survivin expression.