Effect of somatostatin combined with gemcitabine on proliferation of human metastatic pancreatic cancer cell line AsPC-1
LIU Li
RAO Qisuo
LEI Changbin
WANG Wenting
PENG Zhao
KUANG Xingxing
LIU Guowen
Abstract：Objective To observe the effect of combined therapy with somatostatin (SST) and gemcitabine (Gem) on the proliferation of human metastatic pancreatic cancer AsPC-1 cells.Methods The AsPC-1 cells were cultured in vitro.① The cells were treated with 0,100,200,400,600,and 800 μ,g/mL SST for 24,48,and72 h,then we calculated the IC50 of SST.② The cells were treated with 0,0.1,1 5,10,20,40,and 60 μmol/L Gem for 48 h,and we calculated the IC50 of Gem.After that,AsPC-1 cells were divided into four groups:an untreated control group (group A),a group treated with SST at IC50 (group B),a group treated with Gem at IC50 (group C),and a group treated with SST + Gem at half of their IC50 (group D).At 48 h after treatment,the inhibition rates were calculated.Results For AsPC-1 cells,IC50 of SST was 600 μg/mL at 48 h,and IC50 of Gem was 20 μmol/L.The inhibition rates in the groups A,B,C and D were 0.00％ ± 3.79％,52.59％ ± 4.57％,48.11％ ± 10.93％,and 64.86％ ± 4.83％,respectively.The cell growth was significantly inhibited in the groups B,C,and D as compared with that of group A (P ＜ 0.05),and the inhibition rate was significantly higher in the group D than in the groups B and C (P ＜ 0.05).Conclusion The combination of SST and Gem can inhibit the growth of AsPC-1 cells.
Keywords：somatostatingemcitabinemetastatic pancreatic cancer cells AsPC-1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )