Effects of cold stress on sympathetic nerve activity and Stim1 expression of rats with hypertension
XIAO Bing
YANG Xiuchun
LU Jingchao
CHEN Fei
PEI Weina
WANG Xiaozhao
LIU Fan
Abstract：Objective To investigate the effects of cold stress on the sympathetic nerve activity and Stim1 expression of spontaneously hypertensive rats (SHR). Methods Ten male SHR and 10 normal male rats were selected. The normal male rats were randomly divided into the normal control group and cold stress group,and SHR were randomly divided into the SHR model group and SHR cold stress group,with 5 in each. Rats in the normal control group and SHR model group were placed into metabolic cages at room temperature for 6 h,and the rats in the cold stress group and SHR cold stress group were placed into metabolic cages at 4 ℃ for 6 h. Systolic (SBP)and diastolic (DBP)blood pressure was monitored during room temperature and cold stress by tail-cuff method. Urine norepinephrine excretion was detected by HPLC. The mRNA and protein expression of Stim1 in brainstem were detected by quantitative real-time PCR and Western blotting,re-spectively. Results The SBP,DBP and urinary NE excretion were significantly higher in the cold stress group than in the normal control group;the SBP,DBP,and urinary NE excretion were significantly higher in the SHR cold stress group than in the SHR model group and cold stress group. The Stim1 mRNA and protein expression levels in brainstem were signifi-cantly lower in the SHR cold stress group than in the SHR model group and cold stress group (all P < 0. 01). Conclusion In response to cold stress,Stim1 may be the key gene to modulate the sympathetic nerve activity.
Keywords：primary hypertensionstromal interaction molecule 1cold stresssympathetic nerve activity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 27-30 )