Effects of Xihuang pills on proliferation and apoptosis of Wilms tumor

HUANG Yan; WANG Heyi; LI Yuanzhe; LI Yang

doi:10.3969/j.issn.1002-266X.2017.46.003

Abstract：Objective To observe the effects of Xihuang pills on the proliferation and apoptosis of Wilms tumor cells, and to explore its mechanism. Methods We collected the cancer tissues from the diagnosed cancer patients to perform primitive culture. Then,we selected the cells in the logarithmic growth phase to divide them into the high-dose,medium-dose and low-dose groups and the control group which were separately added with 4,2,and 1 mg/ mL water extracts of Xi-huang pills,and the same dose of medium. The proliferation inhibition rate was detected by MTS,the rate of apoptosis was detected by flow cytometry,and the expression of Bax and Bcl-2 proteins was detected by Western blotting. Results The proliferation inhibition rates of the high-dose group,medium-dose group,and low-dose group were higher than that of the control group,and the proliferation inhibition rate of the higher dose group was much higher than that of lower dose group (all P < 0. 05). The apoptosis rates of the high-dose group,medium-dose group,and low-dose group were higher than that of the control group,and the apoptosis rate of the higher dose group was much higher than that of lower dose group (all P< 0. 05). Western blotting showed that the relative expression of Bax was in the following order:high-dose group > medi-um-dose group > low-dose group > control group,all P < 0. 05;and the relative expression of Bcl-2 was in the opposite or-der:high-dose group < medium-dose group < low-dose group < control group,all P < 0. 05. Conclusion Xihuang pills can inhibit the proliferation of Wilms tumor cells and induce its apoptosis,and the mechanism may be related with the up-regulated expression of Bax and down-regulated expression of Bcl-2.