Effects of TGF-β on expression of gastric cancer stem genes Oct4 and Nanog
LI Xin
TUO Biguang
WEN Guorong
JIN Hai
AN Jiaxing
WANG Qianxing
XIE Rui
Abstract：Objective To investigate the influence of transforming growth factor-β (TGF-β)on the expression of gas-tric cancer stem genes Oct4 and Nanog in gastric cancer cells. Methods The cultured gastric cancer cells MKN45 were divided into the control group,TGF-β (10 ng/ mL)group,and TGF-β + BAPTA-AM group. The control group was not treated and the other two groups were stimulated with the corresponding reagent for 24 h. The mRNA and protein expression levels of Nanog and Oct4 were detected by qRT-PCR and Western blotting. High-speed ion imaging system was used to ex-amine the changes of intracellular Ca2 + concentrations in gastric cancer MKN45 cells with normal PSS solution (control group),TGF-β (20 ng/ ml)(TGF-β group)and intracellular calcium chelator BAPTA-AM (BAPTA-AM group). Results Compared with the control group,the mRNA and protein expression levels of Nanog and Oct4 in the TGF-β group were up-regulated (both P < 0. 05);compared with the TGF-β group,the mRNA and protein expression levels of Nanog and Oct4 in the TGF-β + BAPTA-AM group were down-regulated (both P < 0. 05). Compared with the control group,the in-tracellular calcium increased in the TGF-β group (P < 0. 05);compared with the TGF-β group,the intracellular calcium decreased in the BAPTA-AM group (P < 0. 05). Conclusion TGF-β could enhance the expression of cancer stem genes Oct4 and Nanog in gastric cancer cells by increasing the intracellular calcium concentration.
Keywords：gastric carcinomatransforming growth factor-βcytokines of tumor cellsNanogOct4
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )