Expression changes of miRNA-29a/ b/ c and VEGF-A in esophageal squamous cell carcinoma and the correlations between them
SONG Leilei
XU Guangfeng
XIE Hui
ZHANG Naijian
SHAO Qixiang
Abstract：Objective To observe the expression changes of miRNA-29 family (miRNA-29a,miRNA-29b,and miR-NA-29c)and vascular endothelial growth factor-A (VEGF-A)in the esophageal squamous cell carcinoma (ESCC)tissues and to investigate the relationships between them. Methods The expression levels of miRNA-29a/ b/ c and VEGF-A were detected by qRT-PCR and immunohistochemistry (IHC),respectively,in the ESCC tissues and para-carcinoma tissues. The relationship between the expression of miRNA-29a/ b/ c and VEGF-A was further analyzed. We used the bioinformatics method and dual luciferase reporter gene system experiment to predict and verify that miRNA-29a/ b/ c could co-regulate the same target gene VEGF-A. Results The higher expression of VEGF-A and the lower expression of miRNA-29a/ b/ c were detected in the ESCC tissues as compared with that of the para-carcinoma tissues. There was a significant negative correla-tion between the expression levels of miRNA-29a/ b/ c and VEGF-A in ESCC tissues (miRNA-29a:r = - 0. 955,miRNA-29b:r = - 0. 950,miRNA-29c:r = - 0. 893;all P < 0. 01). Bioinformatics analysis showed that VEGF-A could specific-ally bind with miRNA-29a/ b/ c,which could be the common target gene of miRNA-29a/ b/ c. The dual luciferase reporter system validated that VEGF-A was a common target gene of miRNA-29a/ b/ c. Conclusions The miRNA-29a/ b/ c is low expressed,and VEGF-A is highly expressed in the ESCC tissues,and they are negatively correlated with each other. VEGF-A is a common target regulator gene of miRNA-29a/ b/ c.
Keywords：esophageal squamous cell carcinomamiRNA-29amiRNA-29bmiRNA-29cvascular endothelial growth factor-A
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )