Expression of VEGF-C in oral and maxillofacial lymphangioma and its effect on proliferation of human umbilical vein endothelial cells

WANG Guoyun; YANG Xu; XU Tianshu

doi:10.3969/j.issn.1002-266X.2017.44.007

Abstract：Objective To explore the expression of vascular endothelial growth factor-C (VEGF-C) in the occurrence and development of oral and maxillofacial lymphangioma (OML).Methods The tumor tissues (OML group) and the adjacent normal tissue (control group) from OML patients were collected.The real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of VEGF-C mRNA in the tissues.Immunohistochemistry (IHC) was used to detect the positive rate of VEGF-C protein expression in the tissues.ECV304 cells cultured in vitro were randomly divided into two groups:the observation group which was transfected with sh-VEGF-C plasmid and the control group transfected with control plasmid sh-control.After 48-hour transfection,qRT-PCR and Western blotting were used to detect the relative expression of VEGF-C protein,and MTT assay was used to detect the proliferation of the cells (absorbance value).Results In the OML group and control group,the expression of VEGF-C mRNA was 1.061 ± 0.284 and 0.801 ± 0.098,respectively;the positive expression rates of VEGF-C protein was 84.10％ (42/54) and 10.13％ (5/54),respectively (P ＜ 0.05 or P ＜ 0.01).In the observation group and control group,the relative expression levels of VEGF-C mRNA were 0.200 ±0.058 and 0.967 ±0.017,respectively;the relative expression levels of VEGF-C protein were 0.319 ±0.021 and 0.965 ± 0.039,respectively;the A values were 0.393 ± 0.015 and 0.97 ± 0.004,respectively;significant differences were found between these two groups (all P ＜ 0.01).Conclusions VEGF-C is highly expressed in the OML tissues,and decreasing VEGF-C can inhibit the proliferation of human umbilical vein endothelial cells.