Expression changes of miR-106b in non-small-cell lung cancer cell line A549

LI Lei; NI Zhengyi; TANG Zhongwen; ZHOU Mi

doi:10.3969/j.issn.1002-266X.2017.44.006

Abstract：Objective To observe the expression changes of miR-106b in non-small-cell lung cancer (NSCLC) cell line A549,and to investigate its effects on cell migration and invasion,as well as the regulatory mechanism.Methods The expression level of miR-106b was measured in A549 and HPL-1 cells by the fluorescent quantitative PCR.A549 cells were divided into three groups.The miR-106b mimics group was transfected with miR-106b mimics,the miR-106b inhibition group with miR-106b inhibit,and the control group with scramble.The relative expression level of miR-106b was measured by real-time fluorescent quantitative PCR (qRT-PCR) at 24 h after culture.The migration and invasion abilities in the three groups were measured by Scratch test and Transwell invasion assay.The expression levels of FUT6 mRNA and protein were measured in these three groups by fluorescent quantitative PCR and Western blotting,respectively.Results The expression level of miR-106b in the A549 cells was 5.37 ± 0.42,which was higher than that of HPL-1 cells (1.0),P ＜0.01.The expression level of miR-106b was 8.94 ±0.73 in the miR-106b mimics group,0.21 ±0.03 in the miR-106b inhibit group and 1.0 in the control group,respectively,and the difference was significant,all P ＜ 0.05.The wound healing rate and the number of invasive cells in the miR-106b mimics group and control group were significantly higher than those of the miR-106 inhibition group,while the expression levels of FUT6 mRNA and protein were significantly lower than those in the miR-106b inhibit group,and the'change in the miR-106b mimics group was more significance (all P ＜ 0.05).Conclusion The miR-106b is up-regulated in the NSCLC A549 cells and the over-expression of miR-106b promotes the migration and invasion of NSCLC by down-regulating FUT6 expression.