Effect of lidamycin on proliferation of human cervical cancer Caski cells
LU Hua
QIN Ye
ZHAN Jingqiong
WANG Yanlin
Abstract：Objective To investigate the effect of lidamycin on the proliferation of human cervical cancer Caski cells and its underlying molecular mechanisms.Methods Human cervical cancer Caski cells were randomly divided the experimental groups (including the low-dose,medium-dose and high-dose groups) which were separately treated with 1,2.5 and 5 ng/mL lidamycin (100 μL) for 24 h,and the control group which was added with the same volume of RPMI-1640.MTT assay was used to detect the inhibition rate of proliferation.The cell cycle and mitochondrial membrane potential of CasKi cells were measured by flow cytometry.Transmission electronic microscope was used to observe the morphological changes of Caski cells.We calculated the apoptosis rate and mitotic catastrophe rate.Results The proliferation rate and survival rate of the experimental groups were significantly lower than those of the control group,and the decrease in the high-dose group was more significant (all P ＜ 0.05).The percentage of cells in the S phase of the control group and the medium-dose group was higher than those in the low-dose and high-dose groups (all P ＜ 0.05).The percentage of cells in the G0/G1 phase of the experimental groups was lower than that of the control group and the percentage of cells in the G2/M phase was higher than that of the control group,especially in the medium-dose and high-dose groups (all P ＜ 0.05).The ratios of mitochondrial membrane potential decrease in the low-dose,medium-dose and high-dose groups and control group were 34.77％ ± 5.33％,37.87％ ± 5.73％,18.37％ ± 3.91％,and 4.17％ ± 0.65％,respectively.The mitochondrial membrane potential decreased ratios of the experimental groups were higher than that in the control group,especially in the lowdose and medium-dose groups (all P ＜ 0.05).Transmission electronic microscopy showed that the control group had normal morphology while the experimental groups had many forms of death cells,including apoptosis,necrosis and mitotic catastrophe.The apoptosis rate and mitotic catastrophe rate of the experimental groups were both higher than those of control group (both P ＜ 0.05).Conclusion Lidamycin can significantly inhibit the proliferation of Caski cells with an dose-dependent manner by inducing the apoptosis and mitotic catastrophe of cells.
Keywords：cervical carcinomalidamycinhuman cervical cancer Caski cellsapoptosismitotic catastrophemitochondrial membrane potential
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )