Effects of oridonin on killing,proliferation,and expression of NF-κB protein of human glioma U251 cell line
YUAN He yi
WANG Limin
LIU Jun
LIN Dayong
LIU Hong
BAI Jian
ZHANG Liyan
Abstract：Objective To observe effects of oridonin on killing,proliferation,and expression of NF-κB protein of hu-man glioma U251 cell line. Methods U251 cells were incubated with oridonin at the concentrations of 10,20,40,and 80 μmol/ L respectively. At the same time,U251 cells incubated without oridonin were taken as the control group. U251 counting,IC 50 ,U251 cell proliferation inhibition rate and expression of NF-κB were detected at different time points. Re-sults Compared with the control group,the number of U251 cells in the 40 and 80 μmol/ L oridonin groups decreased sig-nificantly (P < 0. 05). After incubation for 24,48 and 72 h,the IC 50 of U251 cells was 82. 2,65. 3 and 44. 1 μmol/ L, respectively. Compared with 20 μmol/ L oridonin group,the cell proliferation inhibition rates of the 40 and 80 μmol/ L ori-donin groups were significantly reduced at 48 and 72 h (P < 0. 05). Compared with the control group,the expression of NF-κB in U251 cells decreased significantly (P < 0. 05)in the 40 and 80 μmol/ L oridonin group at 48 and 72 h. Conclu-sions Oridonin can kill U251 cells,inhibit its proliferation and down-regulate NF-κB protein in vitro. The killing effect and inhibition can be related to the regulation of NF-κB signaling pathway.
Keywords：oridoningliomacell growthcell proliferationNF-κB signaling pathwayNF-κB protein
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 14-16 )