Efficacy of CSCs antigens-loaded dendritic cell vaccine combined with low-dose TP in treatment of breast cancer in mice
CHEN Xin
LIANG Huiling
WU Long
XU Ximing
ZHANG Yiqiao
CHEN Neng
FENG Ling
Abstract：Objective To study the therapeutic effect and mechanism of cancer stem cells (CSCs)antigens-loaded dendritic cell (DC)vaccine combined with low-does TP (paclitaxel and cisplatin)in treatment of breast cancer in mice. Methods We sorted out the CSCs from breast cancer cell lines (4T1)by using ALDEFLUOR. Bone marrow-derived DCs were pulsed with CSCs lysate to generate CSCs-DC vaccine. The Balb/ c mice were inoculated with the heterogeneous 4T1 tumor cells by subcutaneous injection to make the mouse breast cancer models. The models were then divided into four groups:PBS group which was administrated PBS,TP group which was injected with 20 mg/ kg paclitaxel and 5 mg/ kg cis-platin intraperitoneally,CSCs-DC vaccine group (CSCs-DC group)which was administrated CSCs-DC vaccine by subcuta-neous injection,and the CSCs-DC vaccine + TP group (CSCs-DC + TP group)which was administrated CSCs-DC vaccine by subcutaneous injection and was injected with 20 mg/ kg paclitaxel and 5 mg/ kg cisplatin intraperitoneally. Tumor vol-umes were measured and survival was monitored. At the end of the experiment,the spleens in mice were harvested,the cy-totoxic lymphocyte (CTL)activity was measured by using LDH cytotoxicity assay,and IgG levels secreted by B cells was measured by ELISA. Results The tumor size in the CSCs-DC + TP group was less than that of the PBS group,the TP group and the CSCs-DC group (P < 0. 05). The average survival time of the CSCs-DC + TP group was (62. 00 ± 2. 65)d and was the longest,the average survival time of CSCs-DC group was (52. 00 ± 1. 00)d,the average survival time of TP group and PBS group were (46. 00 ± 2. 00)d and (35. 00 ± 1. 00)d,respectively. The CTL activity and IgG levels se-creted by B cells in the CSCs-DC + TP group were the highest,the CSCs-DC group was the second,TP group was lower than the CSCs-DC group and the CSCs-DC + TP group,but was higher than the PBS group,and the difference was statisti-cally significant (P < 0. 01). Conclusion The therapeutic effect of CSCs-DC vaccine combined with low-dose TP in the treatment of the mouse breast cancer is better than CSCs-DC vaccine and TP alone.
Keywords：breast neoplasmscancer stem cellsvccinepaclitaxelcisplatin
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 16-19 )