Expression of FAM96A in human liver cancer tissues and its effect on proliferation and apoptosis of human liver cancer HepG2 cells

LIAO Yusheng; ZHANG Heng; HUANG Manling; TIAN Jun

doi:10.3969/j.issn.1002-266X.2017.41.003

Abstract：Objective To investigate the expression of family with sequence similarity 96 member A(FAM96A)in the liver cancer tissues and to evaluate its effects on the cell proliferation and apoptosis of human liver cancer cells HepG2. Methods Real-time PCR and Western blotting were applied to detect the mRNA and protein expression levels of FAM96A in the liver cancer tissues and their adjacent tissues,HepG2 cells and L02 cells. Furthermore,the recombinant plasmid of pcDNA3. 1-myc-FAM96A containing the whole sequence of human FAM96A gene (FAM96A group)and pcDNA3. 1-vector pcDNA3. 1-vector blank plasmid (control group)were respectively transfected into HepG2 cells. Then MTT essay and flow cytometry were applied to detect the proliferation and apoptosis rate of HepG2 cells in the two groups. Results FAM96A mRNA levels were significantly decreased in the liver tissues than in the adjacent tissues (0. 704 ± 0. 178 vs. 1. 707 ± 0. 267)and in HepG2 cells than in L02 cells (0. 627 ± 0. 251 vs. 1. 654 ± 0. 285),(both P < 0. 05). While FAM96A protein expression levels were lower in the liver tissues as compared with those of the adjacent tissues and were lower in HepG2 cells than in L02 cells (both P < 0. 05). Moreover,MTT assay showed that the proliferation of the FAM96A group was significantly lower than that of the control group (P < 0. 05). FACS analysis indicated that the apoptotic rate was high-er in the FAM96A group than in the control group (33. 06% ± 3. 15% vs. 5. 08% ± 0. 75%),(P < 0. 05). Conclusion The fusion protein SMP30-IP10 eukaryotic expression plasmid is successfully con-structed,and it can inhibit the migration and invasion abilitiesof hepatoma cells,but has no effect on the cell proliferation.