Construction of eukaryotic expression plasmid containing fusion protein SMP30-IP10 and its effect on migration,invasion,and proliferation of liver cancer cells
XIE Meiyu
GUO Shunli
BIN Xiaoyun
SHI Mingxia
CHEN Anning
CHEN Siyu
ZHOU Sufang
Abstract：Objective To construct the eukaryotic expression plasmid of fusion protein SMP30-IP10,and to investi-gate its effects on migration,invasion,and proliferation of hepatoma cell line SK-hep1. Methods The SK-hep1 cells were divided into two groups:the experimental group which was transfected with pIRES-SMP30-IP10 plasmid,and the con-trol group which was transfected with empty pIRES. The eukaryotic expression plasmid pIRES-SMP30-IP10 was constructed by overlapping PCR,and then we transfected the plasimd into hepatoma cell line SK-hep1 by liposome transfection;the protein expression of cells was detected by Western blotting;the migration and invasion of cells were detected by Transwell assay;the proliferation of cells was detected by CCK8. Results The eukaryotic expression plasmid pIRES-SMP30-IP10 was successfully constructed and approved by PCR,double restriction enzyme digestion and DNA sequencing,and was ex-pressed in hepatocellular carcinoma cell line SK-hep1. Compared with the control group,the migration and invasion were inhibited to some extent in the experimental group (both P < 0. 05),but the morphology and proliferation had no significant change (both P > 0. 05). Conclusion The fusion protein SMP30-IP10 eukaryotic expression plasmid is successfully con-structed,and it can inhibit the migration and invasion abilitiesof hepatoma cells,but has no effect on the cell proliferation.
Keywords：liver neoplasmsfusion proteinsenescence marker protein-30interferon-inducible protein 10cell mi-grationcell invasioncell proliferation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )