Effects of hepatitis B virus X protein A1762T / G1764A double mutation on expression of clock gene in liver cancer cells
PENG Hua
ZHAN Jing
ZHANG Yifan
SUN Fei
QIU Mengjun
YANG Shengli
Abstract：Objective To investigate the effects of hepatitis B virus X protein A1762T/ G1764A double mutation on the expression of clock gene in the hepatocellular carcinoma (HCC)cells. Methods Bel-7404 cells were inoculated into into 6-hole plate,when the cells grew to 60% confluence,they were transfected with 1. 0 μg pcDNA3. 1-HBx and pcD-NA3. 1-mHBx (A1762T/ G1764A double mutant)plasmids by using Lipofectamine 2000 according to the manufacturer's in-structions. The mRNA and protein expression levels of the CLOCK,BMAL1,Per1,Per2,Per3,Cry1,Cry2,and CKIεgenes in the Bel-7404 cells were detected by real-time PCR and Western blotting. Results The mRNA and protein expres-sion of Clock,Bmal1,Per2,Per3,Cry1 and Cry2 decreased in Bel-7404 cells transfected with A1762T/ G1764A double mutant HBx vectors as compared with that of cells transfected with pcDNA3. 1-HBx vectors,and the differences were statis-tically significant (all P < 0. 05). Conclusions HBx A1762T/ G1764A double mutation can cause the disorder of biologi-cal clock expression in HCC cells.
Keywords：liver neoplasmshepatitis B virus X proteinmutationcircadian clockclock controlled gene
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )