Effect of CX3CR1 gene knockout on rejection of allogeneic mice after tracheal transplantation

GUAN Xiangqian; LIU Maoni

doi:10.3969/j.issn.1002-266X.2017.40.007

Abstract：Objective To investigate the effect of chemokine receptor CX 3CR1 gene knockout on rejection of alloge-neic mice after tracheal transplantation .Methods Twenty-four mice were divided into three groups: homologous control group, allogenic control group , and gene knockout transplantation group with 4 pairs in each.In the homologous control group, all donor mice and recipient mice were BALB/c mice.In the allogenic control group , donor mice were BALB/c mice, and recipient mice were C57BL/6 male mice.In the gene knockout transplantation group , donor mice were BALB/c mice, and recipient mice were CX3CR1 knockout mice (C57BL/6 background).Trancheal transplantation operation was performed in all groups , and each recipient mouse was transplanted with 2 tracheas.At week 4 after transplantation , the tracheal grafts were removed and the pathological change was detected with HE staining .Meanwhile, the serum high-mobil-ity group box 1 protein (HMGB1) levels in the recipients of each group were detected with ELISA .Results HE staining showed that there was no fibrosis in the tracheal grafts from the homologous control group , and the blocking score was 0.All tracheal grafts from the allogenic control group had fibrosis , and the blocking score was 8.0.In the gene knockout trans-plantation group, 1 tracheal graft had fibrosis, and the blocking score was only 0.96.At week 4 after transplantation, the HMGB1 levels in the homologous control group , allogenic control group , and gene knockout transplantation group were (29.8 ±1.6), (83.6 ±1.4), and (19.8 ±0.9) ng/mL, and the HMGB1 level was lower in the gene knockout trans-plantation group than in the allogenic control group (P<0.05), but no significantly difference was found between the ho-mologous control group and the gene knockout transplantation group (P>0.05).Conclusion Receptor CX3CR1 knock-out can significantly reduce the rejection after tracheal transplantation .