Effects of high glucose and lysophosphatidylcholine and PAF on PKCβI mRNA expression of glomerular mesangial cells and the intervention effect of atorvastatin
XIAO Yanhua
HE Xiaoyun
HAN Qing
HU Xiaowen
ZHOU Suxian
Abstract：Objective To investigate the effect of atorvastatin on the PKCβI mRNA expression of high glucose and lysophosphatidylcholine (LPC)and platelet activating factor (PAF)-induced mesangial cells. Methods Human glomeru-lar mesangial cells (HMCs)were divided into four groups:the normal control group (group A),high glucose and LPC +PAF group (group B),high glucose and LPC + PAF + LY333531 group (group C),and high glucose and high LPC +PAF + atorvastatin group (group D). We added 5. 5 mmol/ L D-glucose to group A;group B was treated with 30 mmol/ L D-glucose,20 mg/ L LPC,and 2 × 10 - 8 mol/ L PAF C-16;group C was pretreated with 2 × 10 - 7 mol/ L LY333531 for 1 h, and then was added with 30 mmol/ L D-glucose,20 mg/ L LPC,and 2 × 10 - 8 mol/ L PAF C-16;group D was pretreated with 10 μmol/ L atorvastatin for 1 h,and then was added with 30 mmol/ L D-glucose,20 mg/ L LPC,2 × 10 - 8 mol/ L PAF C-16. At 24 h after culture,RNA was extracted. The expression of PKCβI mRNA in the mesangial cells was detected by real-time fluorescence quantitative PCR. Results The relative expression of PKCβI-mRNA in the groups A,B,C,and D was 1. 00 ± 0. 00,3. 59 ± 0. 41,2. 76 ± 0. 57,and 1. 02 ± 0. 00,respectively. The expression of PKCβI-mRNA in the group B group was higher than that of the other three groups,and the expression of PKCβI-mRNA in the group D was sig-nificantly lower than that of the groups B and C (all P < 0. 05). Conclusions The expression of PKCβI mRNA in high glucose and LPC and PAF-induced glomerular mesangial cells is up-regulated,and atorvastatin can inhibit the PKCβI ex-pression.
Keywords：glomerular mesangial cellsplatelet-activating factorprotein kinase C-βIatorvastatin
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 27-29 )