Effect of down-regulation of LSINCT5 on proliferation,migration,and radiosensitivity of human glioma cell line U251
FENG Jigao
MO Yehe
XU Pengxiang
HU Dexian
OUYANG Yibin
LIU Dayuan
ZHANG Caicai
Abstract：Objective To investigate the effect of down-regulation of LSINCT5 on proliferation,migration ability,and radiosensitivity of human glioma cell line U251. Methods Lentiviral transfection was used to construct stable LSINCT5 in-terference cells (U251 shLSINCT5 cells),and the cells in the sh LSINCT51# group and sh LSINCT53# group with high efficiency of interference and the cells in the control group (transfected with GV248) were used for the subsequent experi-ments. MTS method was used to detect the changes of cell proliferation abilities at 0,24,48,and 72 h. Scratch healing assay was used to detect the changes of cell migration abilities. Western blotting was used to detect the expression of E-cad-herin,Zo-1 and Vimentin. MTS assay was used to detect the changes of cell proliferation abilities at 0,2,4,8 Gy under X-ray. Results The proliferation abilities of the sh LSINCT51# group and sh LSINCT53# group were significantly lower than that of the control group at 48 and 72 h (all P < 0. 05). The repair distance of the sh LSINCT51# and sh LSINCT53#groups were significantly lower than that of the control group (P < 0. 05). Compared with the control group,the expression levels of E-cadherin and Zo-1 in the sh LSINCT51# group and sh LSINCT53# group increased,and the expression of Vim-entin decreased (all P < 0. 05). The proliferation abilities of sh LSINCT51# group and sh LSINCT53# group were lower than that of the control group at 2 and 4 Gy doses (both P < 0. 05). Conclusion Down-regulation of LSINCT5 expression in human glioma cell line U251 can inhibit cell proliferation and metastasis,and increase radiosensitivity of cells.
Keywords：long non-coding RNA LSINCT5gliomacell proliferationcell migrationepithelial mesenchymal tran-sitionradiosensitivity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 27-30 )