Effects of Stichopus japonicus acid mucopolysaccharide on apoptosis-related protein expression in liver cancer tissues of tumor-bearing mice
LI Xiaojuan
SONg Yang
CHEN Dandan
DAI Haihua
Abstract：Objective To observe the effects of Stichopus japonicus acidic mucopolysaccharide (SJAMP) on apoptosis-related protein expression in liver cancer tissues of tumor-bearing mice.Methods Fifty hepatocarcinoma-bearing mice were established by subcutaneous injection of tumor cells in the right axillary, and then they were randomly divided into the negative control group (injected with normal saline), positive control group (injected with 5-FU 20 mg/kg), low-dose SJAMP group (6.25 mg/kg SJAMP 0.2 mL), medium-dose SJAMP group (12.5 mg/kg SJAMP 0.2 mL), and high-dose SJAMP group (25 mg/kg SJAMP 0.2 mL).The mice were all intervened for 12 days.The mice were sacrificed, and we weighed the tumor and calculated the tumor inhibition rate.HE staining was used to observe the pathological changes of tumor tissues.The expression of Caspase-3, Caspase-9, cytochrome C, Smac protein, survivin and NF-κB was detected by immunohistochemistry.Results The tumor inhibition rate of the high-dose SJAMP group was higher than that of the low-dose and medium-dose SJAMP groups (P<0.05).The decreased cell density and nuclear division as well as increased necrotic area were found in the positive control group, medium-dose and high-dose SJAMP groups.Compared with the negative control group, the expression of Caspase-3, Caspase-9, cytochrome C,and Smac protein increased in the medium-dose, high-dose SJAMP groups and positive control group (P<0.05), while the expression of survivin and NF-κB significantly decreased (all P<0.05).Conclusion The medium-dose and high-dose SJAMP can effectively up-regulate the expression of pro-apoptotic gene, down-regulate the expression of apoptosis inhibiting gene, thus inhibit the growth of tumor-bearing mice.
Keywords：liver carcinomaStichopus japonicus acidic mucopolysaccharideCaspasecytochrome CSmac proteinsurvivinnuclear transcription factor κBmice
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 27-29 )