Effect comparison of small interfering RNA and antisense oligonucleotide in inhibition of RhoA expression of rat hepatic stellate cells
ZHANG Yufeng
YE Kunying
ZHONG Dan
Abstract：Objective To compare the effects of small interfering RNA (siRNA) and antisense oligonucleotide (ASODN) in inhibition of RhoA expression of rat hepatic stellate cells.Methods Rat hepatic stellate cell line HSC-T6 was divided into the siRNA group and ASODN group, respectively, and then each group was separately divided into subgroups A, B, C, and D.Cells in the subgroup A of the siRNA group and ASODN group were cultured normally, subgroup B was transfected with HK-A negative control plasmid, subgroup C with Rat1, and subgroup D with Rat2.After transfection for 48 h, the mRNA expression of RhoA and ColⅠin HSC-T6 cells of each group was detected by reverse-transcription polymerase chain reaction (RT-PCR), respectively;the content of hyaluronic acid (HA) and laminin (LN) in culture serum was measured by specific ELISA.Results The expression of RhoA mRNA and Col I mRNA in the subgroup C of siRNA group and ASODN group was the lowest, and significant difference was found between subgroup A and subgroup C (both P<0.05), but no significant difference was found between the rest of subgroups (all P>0.05).The mRNA expression of RhoA and Col Ⅰ in the subgroup C of the siRNA group was lower than that in the ASODN group (P<0.05).There was no significant difference in the subgroups A, B, and D between the siRNA group and ASODN group (all P>0.05).The levels of HA and LN in the supernate of subgroup C of the siRNA group and ASODN group were the lowest, and significant difference was found between subgroup A and subgroup C (both P<0.05), but no significant difference was found between the rest of subgroups (all P>0.05).The levels of HA and LN in the supernate of subgroup C of the siRNA group were lower than those in the ASODN group (both P<0.05).There was no significant difference in the subgroups A, B, and D between the siRNA group and ASODN group (all P>0.05).Conclusion Both siRNA and ASODN can inhibit RhoA expression of rat HSC-T6 cells, and RNA interference mediated by Rat1 plasmids targeting RhoA can more effectively inhibit the formation of extracellular matrix HA, Col Ⅰ, and LN than that of ASODN.
Keywords：small interfering RNAantisense oligonucleotideRhoAhyaluronic acidcollagenⅠlamininhepatic stellate cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )