Effects of CSF-1R on cyclin D1 expression and cell proliferation of human nasopharyngeal carcinoma 6-10B cells
XU Ximing
CHEN Jiayu
HAO Yanrong
CHEN Jiaxin
HUANG Li
AO Wen
LI Lei
CHENG Junping
CHEN Zhaohong
LIANG Wuqing
HAO Xin
GAO Weiwei
Abstract：Objective To investigate the effects of colony stimulating factor-1 receptor (CSF-1R) on cyclin D1 expression and cell proliferation of human nasopharyngeal carcinoma 6-10B cells.Methods The human nasopharyngeal carcinoma 6-10B cells were transfected with CSF-1R lentiviral vector LV-CSF1R (16957-1) (transfection group), and the 6-10B cells without transfection of CSF-1R lentiviral vector were taken as the control group.The expression of CSF-1R and cyclin D1 was detected by RT-qPCR PCR and Western blotting.CCK-8 assay were used to detect the cell activity in each group.Results The expression of CSF-1R at mRNA and protein levels increased significantly in the transfection group as compared with that of the control group (P<0.05).The activity of 6-10B cells was significantly higher in the transfection group than that of the control group (P<0.05).Conclusion Over-expression of CSF-1R significantly promotes the malignant growth of nasopharyngeal carcinoma 6-10B cells by regulating the expression of cyclin D1.
Keywords：nasopharyngeal carcinomacolony stimulating factor-1 receptorreceptor tyrosine kinasescyclin D1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-22,25 )