Proteomics analysis on serum differential proteins between lower limb arteriosclerosis obliterans patients with and without type 2 diabetes mellitus
YANG Xiaohu
LIN Yuhui
XU Xin
FAN Longhua
Abstract：Objective To identify the differentially expressed proteins between lower limb arteriosclerosis obliterans patients with type 2 diabetes mellitus (DLASO) and patients without type 2 diabetes mellitus (LASO) by the proteomic research.Methods Serum samples were separately collected from DLASO patients and LASO patients (15 cased in each group).Isobaric tags for relative and absolute quantitation (iTRAQ)-tagging combined with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) analysis were used to identify the differentially expressed proteins between DLASO patients and LASO patients.The differentially expressed proteins were analyzed for biological processes, molecular effects and cell composition by using PANTHER database.Results Compared with LASO patients, a total of 27 differentially expressed proteins were identified in DLASO patients, 8 proteins were significantly up-regulated while 19 proteins were significantly down-regulated.Biological process analysis showed that the differential protein was mainly involved in cell reaction (48.1%), metabolic process (48.1%), and bioadhesion (29.6%).The results of molecular analysis showed that the differential protein was mainly involved in cell binding (33.3%), catalytic activity (29.6%), and structural molecule activity (18.5%).Cell composition analysis showed that the differential proteins were mainly located in the cell region (14.8%), organelles, (14.8%) and extracellular regions (14.8%).Conclusion Totally 27 differentially expressed proteins between DLASO and LASO patients are identified by proteomic research, which may be used as potential biological targets for the prevention and treatment of DLASO.
Keywords：type 2 diabetes mellituslower limb arteriosclerosis obliteransproteomicsisotope labellingtwo-dimensional liquid chromatography-tandem mass spectrometrydifferentially expressed proteins
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 18-21 )