Effects of E1A gene on migration and invasion of triple-negative breast cancer cells
TAI Yicheng
FANG Qi
TAN Kelan
HUANG Gui
ZHANG Lingyan
Abstract：Objective To investigate the effects of E1A gene on the migration and invasion of triple-negative breast cancer (TNBC) cells and is mechanism.Methods The eukaryotic expressive vector containing E1A gene (pcDNA3.0-E1A) (E1A stable clone group) and pcDNA3.0 empty plasmid (empty-plasmid clone group) were separately transfected into MDA-MB-231 cells by the lipofectamine, and stable E1A positive clones were selected by G418.Meanwhile, the MDA-MB-231 cells without any transfection were taken as the blank control group.Then Western blotting was utilized to identify the stable clones.The migration and invasion abilities of cells in all groups were analyzed by Scratch test and Transwell assay, respectively.Besides, the protein level of HSPA5 in all groups was detected by Westernblotting.Results MDA-MB-231 cell clones stably transfected with pcDNA3.0-E1A plasmid (E1A clones) were successfully established by the G418.The cell migration and invasion abilities of E1A clones in the E1A stable clone group were much lower as compared with those of the blank control group and the empty-plasmid clone group (P<0.05), accompanied by the down-regulated expression of HSPA5 protein (all P<0.05).Conclusion E1A gene can significantly inhibit the cell invasion and migration of TNBC cells in vitro by E1A promoting the protein degradation of HSPA5.
Keywords：triple-negative breast cancerE1Acell invasioncell migration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 23-25 )