Role of p38 MAPK on apoptosis of mouse embryo fibroblasts induced by MRP8/14
WANG Juan
CHEN Xiaohuan
LI Lei
LU Xiaying
JIANG Yong
Abstract：Objective To explore the role of p38 MAPK on the apoptosis of mouse embryo fibroblasts induced by myeloid-related protein 8 (MRP8)/myeloid-related protein 14 (MRP14).Methods MTT assay was performed to detect the cell viability of p38+/+ and p38-/-cells in the control group and groups in which cells were treated with 50 μg/mL MRP8, MRP14, and MRP8/14 for 24 h and 48 h;flow cytometry was applied to detect the apoptosis rate of p38+/+ and p38-/-cells in the control group and groups in which cells were treated with 50 μg/mL MRP8/14 for 24 h and 48 h;MTT assay was performed to detect the cell viability of p38+/+ cells in the control group, MRP8/14 group, MRP8/14+TAK242(TLR4 inhibitor) group and MRP8/14+RAGE neutralized antibody group for 24 h;MTT and flow cytometry were used to analyze the cell viability and the apoptosis rate of p38+/+ cells in the control group, MRP8/14 group, MRP8/14+SB203580 (p38 MAPK inhibitor) group for 24 h;Western blotting was used to detect the phosphorylation changes of p38 MAPK in p38+/+ cells treated with MRP8/14 for 0, 1, 2, 4, 6, and 8 h, respectively.Results The cell viability of p38+/+ and p38-/-cells in the groups treated with MRP8, MRP14, and MRP8/14 for 24 h and 48 h was lower than that of the control group (P<0.05).At the same time, the cell viability of p38-/-cells was significantly higher than that of p38+/+ cells in the MRP8/14 group at 24 and 48 h (P<0.05).The apoptosis rate of p38+/+ cells in the MRP8/14 group at 24 and 48 h was higher than that of the control group, especially at 48 h (all P<0.05).However, the apoptosis rate of p38-/-cells did not change after MRP8/14 treatment for 24 and 48 h.The cell viability of p38+/+ cells in the MRP8/14 group and MRP8/14+RAGE neutralized antibody group was lower than that in the control group and MRP8/14 +TAK242 group (P<0.05).The cell viability of p38+/+ cells in the MRP8/14+SB203580 group was higher than that of the MRP8/14 alone group, and the apoptosis rate of p38+/+ cells in the MRP8/14+SB203580 group was lower than that of the MRP8/14 alone group (P<0.05).The phosphorylation level of p38 MAPK in p38+/+ cells was significantly higher at 2, 4, and 6 h after MRP8/14 treatment than that at 0, 1, and 8 h (P<0.05).Conclusion MRP8/14 can induce the apoptosis of mouse embryo fibroblasts via the activation of TLR4-p38 MAPK pathway.
Keywords：embryo fibroblastsmyeloid-related protein 8myeloid-related protein 14p38 mitogen-activated protein kinaseapoptosismouse
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 28-32 )