Effects of CD147 on phosphorylation of AR in prostate cancer cells
FANG Fang
ZHAO Chen
HAO Feng
WANG Liguo
Abstract：Objective To investigate the effects of leukocyte differentiation antigen (CD147) on phosphorylation of androgen receptor (AR) in prostate cancer cells and its mechanism.Methods The prostate cancer LNCaP cells were selected and then were randomly divided into the LNCaP/Scramble group and LNCaP/shCD147 group.LNCaP cells in the above two groups were transfected with the negative control virus and CD147 interference virus, respectively.The CD147 expression was detected by Western blotting.The expression of phosphorylated protein kinase B (p-AKT), phosphorylated androgen receptors (p-AR) and prostate specific antigen (PSA) in cells of the two group treated with or without LY294002 was detected by Western blotting.Results The expression of CD147 in the LNCaP/Scramble group was 1.19±0.09, versus 0.71±0.03 in the LNCaP/shCD147 group (P<0.01).We found that the expression of p-AKT, p-AR, and PSA was decreased in the LNCaP/shCD147 group as compared with that of the LNCaP/Scramble group (all P<0.05).When cells were treated with LY294002 for 24 h, the expression of p-AKT, p-AR, and PSA protein was significantly decreased in the LNCaP/Scramble group (all P<0.05).Conclusion CD147 can cause phosphorylation of AR through PI3K/AKT pathway and then increase PSA expression in prostate cancer, which facilitates prostate cancer progress.
Keywords：prostate cancerleukocyte differentiation antigenphosphorylated androgen receptorsphosphorylated protein kinase Bprostate specific antigen
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 9-11 )